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gDNA was extracted from START and END point samples and high-throughput Illumina sequencing was performed to quantify the abundance of each sgRNA by PCR amplification of the sgRNA cassette

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Future research should prioritize the identification of actionable targets within the ferroptotic pathway, assess the regulatory potential of natural compounds and traditional medicines on iron metabolism, and accelerate translational efforts to incorporate ferroptosis-modulating strategies into clinical practice (Table 1)

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After block with 1% bovine serum albumin (BSA) at 4 C for 30 min, the primary antibodies were incubated overnight

Unlike traditional caloric restriction where metabolism slows significantly, Retatrutide users experienced increased energy expenditure from the glucagon receptor activation